Quinolone resistance-determining region in the DNA gyrase gyrA gene of Escherichia coli

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Study of Mutations in the DNA gyrase gyrA Gene of Escherichia coli

Quinolones are a large and widely consumed class of synthetic drugs. Expanded-spectrum quinolones, like ciprofloxacin are highly effective against Gram-negative bacteria, especially Escherichia coli. In E. coli the major target for quinolones is DNA gyrase. This enzyme is composed of two subunits, GyrA and GyrB encoding by gyrA and gyrB, respectively. Mutations in either of these genes cause qu...

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Study of Mutations in the DNA gyrase gyrA Gene of Escherichia coli

Quinolones are a large and widely consumed class of synthetic drugs. Expanded-spectrum quinolones, like ciprofloxacin are highly effective against Gram-negative bacteria, especially Escherichia coli. In E. coli the major target for quinolones is DNA gyrase. This enzyme is composed of two subunits, GyrA and GyrB encoding by gyrA and gyrB, respectively. Mutations in either of these genes cause qu...

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Mutation in the DNA gyrase A Gene of Escherichia coli that expands the quinolone resistance-determining region.

In three Escherichia coli mutants, a change (Ala-51 to Val) in the gyrase A protein outside the standard quinolone resistance-determining region (QRDR) lowered the level of quinolone susceptibility more than changes at amino acids 67, 82, 84, and 106 did. Revision of the QRDR to include amino acid 51 is indicated.

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The involvement of mutation in the serine 83 of quinolone resistant determining regions of the GyrA Gene in resistance to ciprofloxacin in Escherichia coli .

Appearance of bacteria resistant to antibacterial agents puts physicians in trouble and threatens the health of the world. The rapid development of bacterial resistance in Escherichia coli to ciprofloxacin makes difficult the treatment of infectious diseases. So, detection of the locations of possible mutations in gyrase A gene ( gyrA ) in these mutants is very important to determine the mech...

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study of mutations in the dna gyrase gyra gene of escherichia coli

quinolones are a large and widely consumed class of synthetic drugs. expanded-spectrum quinolones, like ciprofloxacin are highly effective against gram-negative bacteria, especially escherichia coli. in e. coli the major target for quinolones is dna gyrase. this enzyme is composed of two subunits, gyra and gyrb encoding by gyra and gyrb, respectively. mutations in either of these genes cause qu...

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ژورنال

عنوان ژورنال: Antimicrobial Agents and Chemotherapy

سال: 1990

ISSN: 0066-4804,1098-6596

DOI: 10.1128/aac.34.6.1271